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SMC1A antibody

Anti-SMC1A antibody used in Immunoprecipitation (IP). GTX113299
Anti-SMC1A antibody used in Western Blot (WB). GTX113299
Anti-SMC1A antibody used in IHC (Paraffin sections) (IHC-P). GTX113299
Anti-SMC1A antibody used in IHC (Paraffin sections) (IHC-P). GTX113299
Anti-SMC1A antibody used in Western Blot (WB). GTX113299
Anti-SMC1A antibody used in IHC (Paraffin sections) (IHC-P). GTX113299

Cat No. GTX113299

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

293T , A431 , H1299 , Molt-4 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMC1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038007

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB

Cellular Localization

Nucleus , Kinetochore

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMC1A antibody used in Immunoprecipitation (IP). GTX113299

GTX113299 IP Image

Immunoprecipitation of SMC1A protein from 293T whole cell extracts using 5 μg of SMC1A antibody (GTX113299).
Western blot analysis was performed using SMC1A antibody (GTX113299) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMC1A antibody used in Western Blot (WB). GTX113299

GTX113299 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Molt-4 (GTX27912)
5% SDS PAGE
GTX113299 diluted at 1:1000

Anti-SMC1A antibody used in IHC (Paraffin sections) (IHC-P). GTX113299

GTX113299 IHC-P Image

Immunohistochemical analysis of paraffin-embedded FaDu Xenograft , using SMC1A(GTX113299) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMC1A antibody used in IHC (Paraffin sections) (IHC-P). GTX113299

GTX113299 IHC-P Image

SMC1A antibody detects SMC1A protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SMC1A antibody (GTX113299) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMC1A antibody used in Western Blot (WB). GTX113299

GTX113299 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX113299 diluted at 1:1000

Anti-SMC1A antibody used in IHC (Paraffin sections) (IHC-P). GTX113299

GTX113299 IHC-P Image

SMC1A antibody detects SMC1A protein at nucleus on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
SMC1A antibody (GTX113299) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for SMC1A antibody (GTX113299). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SMC1A antibody (GTX113299). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169