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SMC3 antibody [C2C3], C-term

Anti-SMC3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110402
Anti-SMC3 antibody [C2C3], C-term used in Western Blot (WB). GTX110402
Anti-SMC3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110402
Anti-SMC3 antibody [C2C3], C-term used in Western Blot (WB). GTX110402
Anti-SMC3 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX110402
Anti-SMC3 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX110402
Anti-SMC3 antibody [C2C3], C-term used in Western Blot (WB). GTX110402

Cat. No. GTX110402

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

PC-12 , HepG2 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SMC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951949

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

structural maintenance of chromosomes 3 , BAM , BMH , CDLS3 , CSPG6 , HCAP , SMC3L1

Cellular Localization

Nucleus

Background

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMC3 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110402

GTX110402 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMC3(GTX110402) antibody at 1:200 dilution.

Anti-SMC3 antibody [C2C3], C-term used in Western Blot (WB). GTX110402

GTX110402 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX110402 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMC3 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110402

GTX110402 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SMC3(GTX110402) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMC3 antibody [C2C3], C-term used in Western Blot (WB). GTX110402

GTX110402 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX110402 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMC3 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX110402

GTX110402 IP Image

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [C2C3], C-term (GTX110402).
Western blot analysis was performed using SMC3 antibody [C2C3], C-term (GTX110402) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMC3 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX110402

GTX110402 IP Image

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [C2C3], C-term (GTX110402) or SMC3 antibody [N1N2], N-term (GTX109827).
Western blot analysis was performed using SMC3 antibody [C2C3], C-term (GTX110402).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMC3 antibody [C2C3], C-term used in Western Blot (WB). GTX110402

GTX110402 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX110402 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169