Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX109827 diluted at 1:1000

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827).
Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: HeLa
7.5% SDS PAGE
GTX109827 diluted at 1:1000

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827) or SMC3 antibody [C2C3], C-term (GTX110402).
Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
5% SDS PAGE
GTX109827 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SMC3 antibody [N1N2], N-term (GTX109827) at 1:300 dilution.