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SMC3 antibody [N1N2], N-term

Anti-SMC3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109827
Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827
Anti-SMC3 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX109827
Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827
Anti-SMC3 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX109827
Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827
Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827

Cat. No. GTX109827

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all SMC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

Mouse liver , A431 , H1299 , HeLa

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SMC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951951

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

structural maintenance of chromosomes 3 , BAM , BMH , CDLS3 , CSPG6 , HCAP , SMC3L1

Cellular Localization

Nucleus

Background

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMC3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109827

GTX109827 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SMC3 antibody [N1N2], N-term (GTX109827) at 1:300 dilution.

Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827

GTX109827 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
5% SDS PAGE
GTX109827 diluted at 1:1000

Anti-SMC3 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX109827

GTX109827 IP Image

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827) or SMC3 antibody [C2C3], C-term (GTX110402).
Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827

GTX109827 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: HeLa
7.5% SDS PAGE
GTX109827 diluted at 1:1000

Anti-SMC3 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX109827

GTX109827 IP Image

Immunoprecipitation of SMC3 protein from HeLa whole cell extracts using 5 μg of SMC3 antibody [N1N2], N-term (GTX109827).
Western blot analysis was performed using SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827

GTX109827 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX109827 diluted at 1:1000

Anti-SMC3 antibody [N1N2], N-term used in Western Blot (WB). GTX109827

GTX109827 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC3 antibody [N1N2], N-term (GTX109827) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169