APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SMC4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886023
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
structural maintenance of chromosomes 4 , CAP-C , CAPC , SMC-4 , SMC4L1
Cellular Localization
Nucleus , Cytoplasm
Background
Members of the structural maintenance of chromosomes, or SMC, family (e.g., SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM]
Database
Research Area
DATA IMAGES
|
GTX129509 IP Image
Immunoprecipitation of SMC4 protein from 293T whole cell extracts using 5 μg of SMC4 antibody (GTX129509).
Western blot analysis was performed using SMC4 antibody (GTX129509) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
GTX129509 WB Image
SMC4 antibody detects SMC4 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
SMC4 antibody (GTX129509) dilution: 1:1000
|
REFERENCE
There are currently no references for SMC4 antibody (GTX129509). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SMC4 antibody (GTX129509). Be the first to share your experience with this product.
![Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624](/upload/website/prouct_img/small/GTX108624/GTX108624_43754_20191108_WB_KO_watermark_20092220_924.jpg "Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624")
