APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SMC4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886023
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
structural maintenance of chromosomes 4 , CAP-C , CAPC , SMC-4 , SMC4L1
Cellular Localization
Nucleus , Cytoplasm
Background
Members of the structural maintenance of chromosomes, or SMC, family (e.g., SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX129509 IP Image
Immunoprecipitation of SMC4 protein from 293T whole cell extracts using 5 μg of SMC4 antibody (GTX129509). Western blot analysis was performed using SMC4 antibody (GTX129509) diluted at 1:1000. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX129509 WB Image
SMC4 antibody detects SMC4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE SMC4 antibody (GTX129509) dilution: 1:1000
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REFERENCE
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REVIEW
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