Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (GTX115669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (GTX115669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.