Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMCR7 antibody [C1C3] (GTX120484) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX120484 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX120484 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.