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SMCR7 antibody

Anti-SMCR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03455
Anti-SMCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX03455
Anti-SMCR7 antibody used in Western Blot (WB). GTX03455
Anti-SMCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX03455
Anti-SMCR7 antibody used in Western Blot (WB). GTX03455

Cat. No. GTX03455

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all SMCR7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MIEF2(Accession Q96C03), corresponding to amino acid residues Y335-V363.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitochondrial elongation factor 2 , MID49 , SMCR7

Cellular Localization

Mitochondrion outer membrane

Background

This gene encodes an outer mitochondrial membrane protein that functions in the regulation of mitochondrial morphology. It can directly recruit the fission mediator dynamin-related protein 1 (Drp1) to the mitochondrial surface. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-SMCR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03455

GTX03455 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX03455 SMCR7 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-SMCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX03455

GTX03455 IHC-P Image

IHC-P analysis of rat muscle tissue using GTX03455 SMCR7 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-SMCR7 antibody used in Western Blot (WB). GTX03455

GTX03455 WB Image

WB analysis of HT-1080 whole cell lysate using GTX03455 SMCR7 antibody.

Anti-SMCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX03455

GTX03455 IHC-P Image

IHC-P analysis of rat skin tissue using GTX03455 SMCR7 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-SMCR7 antibody used in Western Blot (WB). GTX03455

GTX03455 WB Image

WB analysis of various sample lysates using GTX03455 SMCR7 antibody.
Lane 1 : 3T3 whole cell lysate treated with blocking peptide
Lane 2 : 3T3 whole cell lysate
Lane 3 : COS-7 whole cell lysate
Lane 4 : MCF7 whole cell lysate

REFERENCE

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REVIEW

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Package List Price ($)
$ 399