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SMCR8 antibody [HL1007]

Recombinant antibody
Anti-SMCR8 antibody [HL1007] used in IHC (Paraffin sections) (IHC-P). GTX635798
Anti-SMCR8 antibody [HL1007] used in Western Blot (WB). GTX635798
Anti-SMCR8 antibody [HL1007] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635798
Anti-SMCR8 antibody [HL1007] used in Western Blot (WB). GTX635798
Anti-SMCR8 antibody [HL1007] used in Western Blot (WB). GTX635798
Anti-SMCR8 antibody [HL1007] used in IHC (Paraffin sections) (IHC-P). GTX635798

Cat. No. GTX635798

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1007

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMCR8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMCR8. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888578

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMCR8-C9orf72 complex subunit

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-SMCR8 antibody [HL1007] used in IHC (Paraffin sections) (IHC-P). GTX635798

GTX635798 IHC-P Image

SMCR8 antibody [HL1007] detects SMCR8 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SMCR8 stained by SMCR8 antibody [HL1007] (GTX635798) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMCR8 antibody [HL1007] used in Western Blot (WB). GTX635798

GTX635798 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMCR8 antibody [HL1007] (GTX635798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMCR8 antibody [HL1007] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635798

GTX635798 ICC/IF Image

SMCR8 antibody [HL1007] detects SMCR8 protein at nucleus by immunofluorescent analysis.
Sample: DIV10 rat E19 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMCR8 stained by SMCR8 antibody [HL1007] (GTX635798) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.

Anti-SMCR8 antibody [HL1007] used in Western Blot (WB). GTX635798

GTX635798 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMCR8 antibody [HL1007] (GTX635798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMCR8 antibody [HL1007] used in Western Blot (WB). GTX635798

GTX635798 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMCR8 antibody [HL1007] (GTX635798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMCR8 antibody [HL1007] used in IHC (Paraffin sections) (IHC-P). GTX635798

GTX635798 IHC-P Image

SMCR8 antibody [HL1007] detects SMCR8 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SMCR8 stained by SMCR8 antibody [HL1007] (GTX635798) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Bauer CS et al. Acta Neuropathol 2022; 144 (3) : 437-464 An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations