Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMCR8 antibody (GTX134771) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMCR8 antibody (GTX134771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SMCR8 antibody detects SMCR8 protein at nucleus by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMCR8 stained by SMCR8 antibody (GTX134771) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.

SMCR8 antibody detects SMCR8 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMCR8 stained by SMCR8 antibody (GTX134771) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).