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SMG7 antibody

Anti-SMG7 antibody used in Western Blot (WB). GTX121320
Anti-SMG7 antibody used in Western Blot (WB). GTX121320
Anti-SMG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX121320

Cat No. GTX121320

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMG7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164524

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMG7 nonsense mediated mRNA decay factor , C1orf16 , EST1C , SGA56M

Cellular Localization

Cytoplasm , Nucleus

Background

SMG7 is involved in nonsense-mediated mRNA decay (Fukuhara et al., 2005 [PubMed 15721257]).[supplied by OMIM]

Database

DATA IMAGES

Anti-SMG7 antibody used in Western Blot (WB). GTX121320

GTX121320 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMG7 antibody (GTX121320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMG7 antibody used in Western Blot (WB). GTX121320

GTX121320 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMG7 antibody (GTX121320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMG7 antibody used in IHC (Paraffin sections) (IHC-P). GTX121320

GTX121320 IHC-P Image

SMG7 antibody detects SMG7 protein at cytoplasm and nucleus in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SMG7 antibody (GTX121320) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169