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SMG7 antibody

Anti-SMG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87360
Anti-SMG7 antibody used in Western Blot (WB). GTX87360

Cat No. GTX87360

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human SMG7 (521-570).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C1orf16 , EST1C , SGA56M , SMG7 , SMG7, nonsense mediated mRNA decay factor , SMG7 nonsense mediated mRNA decay factor

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

Database

DATA IMAGES

Anti-SMG7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87360

GTX87360 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX87360 SMG7 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-SMG7 antibody used in Western Blot (WB). GTX87360

GTX87360 WB Image

WB analysis of HeLa, Jurkat, 293, and HUVEC cell lysates using GTX87360 SMG7 antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359