APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Rat, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SMNDC1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10722700
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
survival motor neuron domain containing 1 , SMNR , SPF30 , TDRD16C
Cellular Localization
Nucleus speckle , Nucleus , Cajal body
Background
This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/templates/default/js/lightbox/images/loading.gif "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
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GTX118263 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using SMNDC1(GTX118263) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118263 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using SMNDC1(GTX118263) antibody at 1:500 dilution.
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GTX118263 IHC-P Image
SMNDC1 antibody [N1C3-2] detects SMNDC1 protein at nucleus in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SMNDC1 antibody [N1C3-2] (GTX118263) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX118263 IHC-P Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMNDC1(GTX118263) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118263 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SMNDC1 antibody [N1C3-2] (GTX118263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for SMNDC1 antibody [N1C3-2] (GTX118263). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SMNDC1 antibody [N1C3-2] (GTX118263). Be the first to share your experience with this product.
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/upload/website/prouct_img/small/GTX118263/GTX118263_40415_IHC_w_23060519_480.webp "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118263](/upload/website/prouct_img/small/GTX118263/GTX118263_40415_IFA_w_23060519_930.webp "Anti-SMNDC1 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/upload/website/prouct_img/small/GTX118263/GTX118263_40415_20150724_IHC-P_w_23060519_482.webp "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/upload/website/prouct_img/small/GTX118263/GTX118263_40415_IHC_M_w_23060519_942.webp "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in Western Blot (WB). GTX118263](/upload/website/prouct_img/small/GTX118263/GTX118263_40415_20181228_WB_w_23060519_896.webp "Anti-SMNDC1 antibody [N1C3-2] used in Western Blot (WB). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/upload/website/prouct_img/normal/GTX118263/GTX118263_40415_IHC_w_23060519_480.webp "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118263](/upload/website/prouct_img/normal/GTX118263/GTX118263_40415_IFA_w_23060519_930.webp "Anti-SMNDC1 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/upload/website/prouct_img/normal/GTX118263/GTX118263_40415_20150724_IHC-P_w_23060519_482.webp "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263](/upload/website/prouct_img/normal/GTX118263/GTX118263_40415_IHC_M_w_23060519_942.webp "Anti-SMNDC1 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX118263")
![Anti-SMNDC1 antibody [N1C3-2] used in Western Blot (WB). GTX118263](/upload/website/prouct_img/normal/GTX118263/GTX118263_40415_20181228_WB_w_23060519_896.webp "Anti-SMNDC1 antibody [N1C3-2] used in Western Blot (WB). GTX118263")