Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMOC1 antibody [N2C3] (GTX119208) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMOC1(GTX119208) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min