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SMPD4 antibody

Anti-SMPD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX120642
Anti-SMPD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX120642
Anti-SMPD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX120642

Cat No. GTX120642

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMPD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885505

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sphingomyelin phosphodiesterase 4 , NET13 , NSMASE-3 , NSMASE3 , SKNY

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein , Golgi apparatus membrane; Single-pass membrane protein

Background

The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017]

Database

Research Area

DATA IMAGES

Anti-SMPD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX120642

GTX120642 IHC-P Image

SMPD4 antibody detects SMPD4 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
SMPD4 antibody (GTX120642) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMPD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX120642

GTX120642 IHC-P Image

SMPD4 antibody detects SMPD4 protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
SMPD4 antibody (GTX120642) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMPD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX120642

GTX120642 IHC-P Image

SMPD4 antibody detects SMPD4 protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
SMPD4 antibody (GTX120642) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169