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SMRT antibody [1212]

Cat No. GTX70246

Host

Mouse

Clonality

Monoclonal

Clone Name

1212

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr, IP, ChIP assay

Reactivity

Human, Monkey
Package
100 μl ($329)

APPLICATION

Application Note

For ChIP: Use 3μl for 10^6 cells. For IHC-Fr: Use at a dilution of 1:50. Immunohistochemical staining of SMRT in HeLa cells with this antibody results in nuclear staining. For WB: Use at a dilution of 1:250. Detects a band of approximately 220-250 kDa (predicted molecular weight: 274 kDa). Optimal dilutions/concentration should be determined by the researcher.

Calculated MW

168 kDa. ( Note )

Positive Control

HBL100

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffererd saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide from the human SMRT protein corresponding to amino acids 994-1005 (ASASDPHREKTQ) coupled to KLH.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Nuclear Receptor Corepressor 2 , Ctg26 , N-Cor2 , Smap270 , Smrt , Smrte , Smrte-Tau , Tnrc14 , Trac , Trac-1 , Trac1 , Ncor2

Background

Clone 1212 recognizes the Silencing Mediator for Retinoid and Thyroid receptors (SMRT) gene product, a 168 kDa nuclear protein. Nuclear hormone receptors are transcription factors regulated by ligand binding. In the absence of ligand there is a physical interaction between the nuclear receptors that do not interact with Hsp90 and a family of corepressors which includes SMRT. SMRT helps target histone deacetylases and other components of a multiprotein repressor complex to the specific target promoter to silence gene transcription.

Database

Research Area

REFERENCE

REVIEW

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Package List Price ($)
$ 329