GeneTex

  • Country / Location Selection

United States (US)

SMRT antibody [1212]

KO/KD

Cat. No. GTX70246

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1212

Isotype

 IgG1

Application

 WB, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Monkey
Package
100 μl ($399)
See all SMRT products

APPLICATION

Application Note

For ChIP: Use 3μl for 10^6 cells. For IHC-Fr: Use at a dilution of 1:50. Immunohistochemical staining of SMRT in HeLa cells with this antibody results in nuclear staining. For WB: Use at a dilution of 1:250. Detects a band of approximately 220-250 kDa (predicted molecular weight: 274 kDa). Optimal dilutions/concentration should be determined by the researcher.

Calculated MW

274 kDa. ( Note )

Positive Control

HBL100

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide from the human SMRT protein corresponding to amino acids 994-1005 (ASASDPHREKTQ) coupled to KLH.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_373013

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor corepressor 2 , CTG26 , N-CoR2 , SMAP270 , SMRT , SMRTE , SMRTE-tau , TNRC14 , TRAC , TRAC-1 , TRAC1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011]

Database

Research Area

REFERENCE

Application Reference

Fabre MS et al. PLoS One 2020; 15 (4) : e0231470 The oncogene BCL6 is up-regulated in glioblastoma in response to DNA damage, and drives survival after therapy.
Application : WB
Reactivity : Human
Pondugula SR et al. Drug Metab Dispos 2009; 37 (4) : 719-30 A phosphomimetic mutation at threonine-57 abolishes transactivation activity and alters nuclear localization pattern of human pregnane x receptor.
Application : WB
Reactivity : Human, Monkey
Johnson DR et al. Mol Pharmacol 2006; 69 (1) : 99-108 Regulation and binding of pregnane X receptor by nuclear receptor corepressor silencing mediator of retinoid and thyroid hormone receptors (SMRT).
Reactivity : Monkey
Submit a Reference

REVIEW

There are currently no reviews for SMRT antibody [1212] (GTX70246). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
Custom Conjugations