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SMURF1 antibody

Anti-SMURF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17223
Anti-SMURF1 antibody used in Western Blot (WB). GTX17223

Cat. No. GTX17223

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SMURF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HEK293 Cell Lysate(GTX27902)

Product Note

At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1.The immunogen is located within amino acids 180 - 230 of SMURF1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD specific E3 ubiquitin protein ligase 1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-SMURF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17223

GTX17223 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX17223 SMURF1 antibody.
Working concentration : 5 μg/ml

Anti-SMURF1 antibody used in Western Blot (WB). GTX17223

GTX17223 WB Image

WB analysis of 293 cell lysate using GTX17223 SMURF1 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399