Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using SMYD1 antibody(GTX119484) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using SMYD1 antibody(GTX119484) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMYD1(GTX119484) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX119484 diluted at 1:10000

Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX119484 diluted at 1:10000