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SMYD2 antibody

Anti-SMYD2 antibody used in Western Blot (WB). GTX116510
Anti-SMYD2 antibody used in Immunoprecipitation (IP). GTX116510
Anti-SMYD2 antibody used in Western Blot (WB). GTX116510
Anti-SMYD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116510

Cat. No. GTX116510

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all SMYD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa , HCT116

Product Note

Based on sequence homology, this antibody may cross react with other family members.

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMYD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET and MYND domain containing 2 , HSKM-B , KMT3C , ZMYND14

Background

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SMYD2 antibody used in Western Blot (WB). GTX116510

GTX116510 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD2 antibody (GTX116510) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMYD2 antibody used in Immunoprecipitation (IP). GTX116510

GTX116510 IP Image

Immunoprecipitation of SMYD2 protein from HeLa whole cell extracts using 5 μg of SMYD2 antibody (GTX116510).
Western blot analysis was performed using SMYD2 antibody (GTX116510).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMYD2 antibody used in Western Blot (WB). GTX116510

GTX116510 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: HCT116
10% SDS PAGE
GTX116510 diluted at 1:1000

Anti-SMYD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116510

GTX116510 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SMYD2(GTX116510) antibody at 1:200 dilution.

REFERENCE

Application Reference

Li LX et al. Sci Adv 2020; 6 (44) Cross-talk between CDK4/6 and SMYD2 regulates gene transcription, tubulin methylation, and ciliogenesis.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169