SMYD3 antibody [HL2464]

SMYD3 antibody [HL2464] detects SMYD3 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole zebrafish extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.