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SMYD3 antibody

KO/KD Overexpression
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in Immunoprecipitation (IP). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in ChIP assay (ChIP assay). GTX121945
Anti-SMYD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

Cat. No. GTX121945

Reference 6
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SMYD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

DDDDK-tagged SMYD3-transfected 293T

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721057

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET and MYND domain containing 3 , KMT3E , ZMYND1 , ZNFN3A1 , bA74P14.1

Cellular Localization

Cytoplasm , Nucleus

Background

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMYD3 antibody used in Immunoprecipitation (IP). GTX121945

GTX121945 IP Image

Immunoprecipitation of SMYD3 protein from 293T whole cell extracts using 5 μg of SMYD3 antibody (GTX121945).
Western blot analysis was performed using SMYD3 antibody (GTX121945) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMYD3 antibody used in ChIP assay (ChIP assay). GTX121945

GTX121945 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..

Anti-SMYD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121945

GTX121945 ICC/IF Image

SMYD3 antibody detects SMYD3 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: SMYD3 protein stained by SMYD3 antibody (GTX121945) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

SMYD3 antibody detects SMYD3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at a dilution of 1:1000.

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

KO/KD

The data was published in the journal Sci Rep in 2017. PMID: 28630472

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

KO/KD Overexpression

The data was published in the journal Sci Rep in 2017. PMID: 28630472

REFERENCE

Application Reference

Sun J et al. J Hepatocell Carcinoma 2023; 10 : 1237-1256 A Positive Feedback Loop of lncRNA HOXD-AS2 and SMYD3 Facilitates Hepatocellular Carcinoma Progression via the MEK/ERK Pathway.
Fittipaldi R et al. Cells 2021; 10 (5) The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development.
Application : WB
Reactivity : Human
Yang D et al. Aging (Albany NY) 2020; 12 (21) : 21423-21445 Smyd3-PARP16 axis accelerates unfolded protein response and vascular aging.
Application : IP
Reactivity : Mouse
Fenizia C et al. Nucleic Acids Res 2019; 47 (3) : 1278-1293 SMYD3 promotes the epithelial-mesenchymal transition in breast cancer.
Chen YJ et al. Sci Rep 2017; 7 (1) : 3842 SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression.
Application : WB
Reactivity : Human
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REVIEW

SMYD3 antibody Cat. No. GTX121945

Rating
( Average 5 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-SMYD3 antibody used in Immunoprecipitation (IP). GTX121945
Date : Anonymous submitted on 19-Oct-2012
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Amount used : 0.5,1,2mg each for IP
Blocking : 5% non-fat milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Figure Note : 5ug for IP
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169