GeneTex
  • Country / Location Selection

United States (US)

SMYD3 antibody

Anti-SMYD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945
Anti-SMYD3 antibody used in Immunoprecipitation (IP). GTX121945
Anti-SMYD3 antibody used in ChIP assay (ChIP assay). GTX121945
Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

Cat No. GTX121945

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

DDDDK-tagged SMYD3-transfected 293T , 293T , A431 , HeLa , HepG2 , A549 , SKOV3 , HCT116

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721057

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SET and MYND domain containing 3 , KMT3E , ZMYND1 , ZNFN3A1 , bA74P14.1

Cellular Localization

Cytoplasm , Nucleus

Background

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SMYD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121945

GTX121945 ICC/IF Image

SMYD3 antibody detects SMYD3 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min.
Green: SMYD3 protein stained by SMYD3 antibody (GTX121945) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

SMYD3 antibody detects SMYD3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at a dilution of 1:1000.

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMYD3 antibody used in Immunoprecipitation (IP). GTX121945

GTX121945 IP Image

Immunoprecipitation of SMYD3 protein from 293T whole cell extracts using 5 μg of SMYD3 antibody (GTX121945).
Western blot analysis was performed using SMYD3 antibody (GTX121945) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMYD3 antibody used in ChIP assay (ChIP assay). GTX121945

GTX121945 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..

Anti-SMYD3 antibody used in Western Blot (WB). GTX121945

GTX121945 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SMYD3 antibody Cat No. GTX121945

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28630472
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28630472
Date : Anonymous submitted on 19-Oct-2012
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Amount used : 0.5,1,2mg each for IP
Blocking : 5% non-fat milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Figure Note : 5ug for IP
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf