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SMYD5 antibody [C2C3], C-term

Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518
Anti-SMYD5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110518
Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518
Anti-SMYD5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110518
Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518

Cat No. GTX110518

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7)

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SMYD5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951954

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518

GTX110518 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD5 antibody [C2C3], C-term (GTX110518) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMYD5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110518

GTX110518 IHC-P Image

SMYD5 antibody [C2C3], C-term detects SMYD5 protein at cytoplasm and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
SMYD5 antibody [C2C3], C-term (GTX110518) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518

GTX110518 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
10% SDS PAGE
GTX110518 diluted at 1:1000

Anti-SMYD5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110518

GTX110518 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SMYD5(GTX110518) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMYD5 antibody [C2C3], C-term used in Western Blot (WB). GTX110518

GTX110518 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX110518 diluted at 1:1000

REFERENCE

There are currently no references for SMYD5 antibody [C2C3], C-term (GTX110518). Be the first to share your publications with this product.

REVIEW

SMYD5 antibody [C2C3], C-term Cat No. GTX110518

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5%milk in TBST
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169