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SNAI1 antibody [HL2303]

Recombinant antibody KO/KD Orthogonal
Anti-SNAI1 antibody [HL2303] used in Western Blot (WB). GTX638370
Anti-SNAI1 antibody [HL2303] used in Western Blot (WB). GTX638370
Anti-SNAI1 antibody [HL2303] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638370

Cat. No. GTX638370

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2303

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SNAI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SNAI1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

snail family transcriptional repressor 1 , SLUGH2 , SNA , SNAH , SNAIL , SNAIL1 , dJ710H13.1

Cellular Localization

Nucleus,Cytoplasm

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SNAI1 antibody [HL2303] used in Western Blot (WB). GTX638370

GTX638370 WB Image

KO/KD

Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (GTX638370) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SNAI1 antibody [HL2303] used in Western Blot (WB). GTX638370

GTX638370 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (GTX638370) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody [HL2303] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638370

GTX638370 ICC/IF Image

Orthogonal

SNAI1 antibody [HL2303] detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 stained by SNAI1 antibody [HL2303] (GTX638370) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations