GeneTex
United States (US)

SNAI1 antibody

GTX125918_41598_20171215_WB_treat_TGF-beta_EGF_competitor_watermark_18121410_120.jpg
GTX125918_41703_20181221_WB_19021719_121.jpg
GTX125918_41598_20161027_WB_TGF-beta_EGF_18121410_634.jpg
GTX125918_41598_20161027_IFA_18121410_396.jpg
GTX125918_41598_IHC_18121410_657.jpg

Cat No. GTX125918

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa , Hela (1 ng/ml TGF-beta and 300 ng/ml of EGF for 2 hr) , HeLa (starvation for 16 hr, 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min)

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Snail Family Transcriptional Repressor 1,Slugh2,Sna,Snah,Snail,Snail1,Dj710H13.1,Snai1

Cellular Localization

Nucleus , Cytoplasm

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX125918_41598_20171215_WB_treat_TGF-beta_EGF_competitor_watermark_18121410_120.jpg

GTX125918 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX125918) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX125918_41703_20181221_WB_19021719_121.jpg

GTX125918 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX125918_41598_20161027_WB_TGF-beta_EGF_18121410_634.jpg

GTX125918 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125918_41598_20161027_IFA_18121410_396.jpg

GTX125918 ICC/IF Image

SNAI1 antibody detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 protein stained by SNAI1 antibody (GTX125918) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX125918_41598_IHC_18121410_657.jpg

GTX125918 IHC-P Image

SNAI1 antibody detects SNAI1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SNAI1 antibody (GTX125918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169