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SNAI1 (phospho Ser246) antibody

Anti-SNAI1 (phospho Ser246) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55326
Anti-SNAI1 (phospho Ser246) antibody used in Western Blot (WB). GTX55326

Cat. No. GTX55326

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all SNAI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 246(T-F-S(p)-R-M) derived from human SNAI1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

snail family transcriptional repressor 1 , SLUGH2 , SNA , SNAH , SNAIL , SNAIL1 , dJ710H13.1

Cellular Localization

Nucleus,Cytoplasm

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SNAI1 (phospho Ser246) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55326

GTX55326 ICC/IF Image

ICC/IF analysis of methanol-fixed HUVEC cells using GTX55326 SNAI1 (phospho Ser246) antibody.

Anti-SNAI1 (phospho Ser246) antibody used in Western Blot (WB). GTX55326

GTX55326 WB Image

WB analysis of extracts from HT-29 cells using GTX55326 SNAI1 (phospho Ser246) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399