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SNAP25 antibody [4E11]

Anti-SNAP25 antibody [4E11] used in Western Blot (WB). GTX57559
Anti-SNAP25 antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57559
Anti-SNAP25 antibody [4E11] used in Flow cytometry (FACS). GTX57559
Anti-SNAP25 antibody [4E11] used in Western Blot (WB). GTX57559

Cat. No. GTX57559

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E11

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SNAP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 4E11 is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNAP27 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptosome associated protein 25 , CMS18 , RIC4 , SEC9 , SNAP , SNAP25 , SUP , bA416N4.2 , dJ1068F16.2

Cellular Localization

Cytoplasm,Perinuclear region,Cell membrane,Cell junction,Synapse,Synaptosome

Background

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SNAP25 antibody [4E11] used in Western Blot (WB). GTX57559

GTX57559 WB Image

WB analysis of Mouse brain tissue lysate using GTX57559 SNAP25 antibody.
Loading : 30 μg
Dilution : 1:2000

Anti-SNAP25 antibody [4E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57559

GTX57559 ICC/IF Image

ICC/IF analysis of U87-MG cells using GTX57559 SNAP25 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-SNAP25 antibody [4E11] used in Flow cytometry (FACS). GTX57559

GTX57559 FACS Image

FACS analysis of U87-MG cells using GTX57559 SNAP25 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-SNAP25 antibody [4E11] used in Western Blot (WB). GTX57559

GTX57559 WB Image

WB analysis of various samples using GTX57559 SNAP25 antibody.
Lane 1 : 293T cell lysate
Lane 2 : SNAP25 transfected 293T cell lysate
Loading : 10 μg
Dilution : 1:3000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399