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SNAP25 antibody, C-term

Anti-SNAP25 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89577
Anti-SNAP25 antibody, C-term used in Western Blot (WB). GTX89577

Cat. No. GTX89577

Reference 4

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all SNAP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.01-0.03μg/ml
IHC-P 1-2μg/ml
IHC Assay dependent

Note :

IHC-P
Human Cerebellum shows strong axon staining, particularly in the granular region.
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89577-PEP).

Product Note

This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B).

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DEANQRATKMLGSG, from the C Terminus of the protein sequence according to NP_003072.2; NP_570824.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptosome associated protein 25 , CMS18 , RIC-4 , RIC4 , SEC9 , SNAP , SNAP-25 , SUP , bA416N4.2 , dJ1068F16.2

Cellular Localization

Cell membrane

Background

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SNAP25 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89577

GTX89577 IHC-P Image

IHC-P analysis of human cerebellum using GTX89577 SNAP25 antibody, C-term.
Antigen retrieval : citrate buffer Ph 6
Dilution : 1μg/ml

Anti-SNAP25 antibody, C-term used in Western Blot (WB). GTX89577

GTX89577 WB Image

WB analysis of human brain (cerebellum) (A) and mouse brain (B lysates using GTX89577 SNAP25 antibody, C-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Larson ED et al. eNeuro 2020; 7 (1) Function, Innervation, and Neurotransmitter Signaling in Mice Lacking Type-II Taste Cells.
Wilson CE et al. eNeuro 2019; 6 (2) Physiological and Behavioral Responses to Optogenetic Stimulation of PKD2L1+ Type III Taste Cells.
Wilson CE et al. Chem Senses 2017; 42 (9) : 759-767 Type III Cells in Anterior Taste Fields Are More Immunohistochemically Diverse Than Those of Posterior Taste Fields in Mice.
Wilson C. E. et al. Chemical Senses 2017; (Epub) Type III cells in anterior taste fields are more immunohistochemically diverse than those of posterior taste fields in mice.
Application : IHC
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399