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SNAP25 antibody

Anti-SNAP25 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113839
Anti-SNAP25 antibody used in Western Blot (WB). GTX113839
Anti-SNAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113839
Anti-SNAP25 antibody used in Western Blot (WB). GTX113839
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839
Anti-SNAP25 antibody used in Western Blot (WB). GTX113839
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

Cat No. GTX113839

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNAP25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

synaptosome associated protein 25,CMS18,RIC-4,RIC4,SEC9,SNAP,SNAP-25,SUP,bA416N4.2,dJ1068F16.2

Cellular Localization

Cytoplasm , perinuclear region , Cell membrane; Lipid-anchor , Cell junction , synapse , synaptosome

Background

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SNAP25 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113839

GTX113839 IHC-Fr Image

SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SNAP25 antibody used in Western Blot (WB). GTX113839

GTX113839 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX113839 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113839

GTX113839 ICC/IF Image

SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SNAP25 antibody used in Western Blot (WB). GTX113839

GTX113839 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113839 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

GTX113839 IHC-P Image

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

GTX113839 IHC-P Image

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAP25 antibody used in Western Blot (WB). GTX113839

GTX113839 WB Image

SNAP25 antibody detects SNAP25 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
12% SDS-PAGE
SNAP25 antibody (GTX113839) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

GTX113839 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SNAP25(GTX113839) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169