SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SNAP25(GTX113839) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SNAP25 antibody detects SNAP25 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: SNAP25 stained by SNAP25 antibody (GTX113839) diluted at 1:50.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Front Neurol in 2014. PMID: 25002857