SNAP25 antibody

SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SNAP25(GTX113839) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SNAP25 antibody detects SNAP25 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: SNAP25 stained by SNAP25 antibody (GTX113839) diluted at 1:50.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

The data was published in the journal Front Neurol in 2014. PMID: 25002857