Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP29 antibody (GTX131028) diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP29 antibody (GTX131028) diluted at 1:1000.

SNAP29 antibody detects SNAP29 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAP29 protein stained by SNAP29 antibody (GTX131028) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).