APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.68 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SNF8. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618073
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
SNF8 subunit of ESCRT-II , Dot3 , EAP30 , VPS22
Cellular Localization
Cytoplasm (Probable) , Nucleus
Background
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX119948 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
GTX119948 diluted at 1:1000
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GTX119948 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SNF8(GTX119948) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX119948 IHC-Wm Image
SNF8 antibody detects Snf8 protein on blood cells by immunohistochemical analysis Sample: Whole-mount zebrafish embryo SNF8 antibody (GTX119948) dilution: 1:200.
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REFERENCE
There are currently no references for SNF8 antibody (GTX119948). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SNF8 antibody (GTX119948). Be the first to share your experience with this product.
