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SNRK antibody [N3C2], Internal

Anti-SNRK antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111380
Anti-SNRK antibody [N3C2], Internal used in Western Blot (WB). GTX111380
Anti-SNRK antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111380
Anti-SNRK antibody [N3C2], Internal used in Western Blot (WB). GTX111380

Cat No. GTX111380

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , H1299 nuclear extract

Predict Reactivity

Rat, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNRK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SNF related kinase , HSNFRK

Cellular Localization

Nucleus

Background

SNRK is a member of the sucrose nonfermenting (SNF)-related kinase family of serine/threonine kinases (Kertesz et al., 2002 [PubMed 12234663]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SNRK antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111380

GTX111380 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using SNRK(GTX111380) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNRK antibody [N3C2], Internal used in Western Blot (WB). GTX111380

GTX111380 WB Image

SNRK antibody [N3C2], Internal detects SNRK protein by Western blot analysis. H1299 whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SNRK antibody [N3C2], Internal (GTX111380) diluted at 1:500.

Anti-SNRK antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111380

GTX111380 ICC/IF Image

SNRK antibody [N3C2], Internal detects SNRK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNRK protein stained by SNRK antibody [N3C2], Internal (GTX111380) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-SNRK antibody [N3C2], Internal used in Western Blot (WB). GTX111380

GTX111380 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SNRK antibody [N3C2], Internal (GTX111380) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SNRK antibody [N3C2], Internal Cat No. GTX111380

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : KARTHIKEYAN THIRUGNANAM submitted on 20-Nov-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse HL1 atrial cardiomyocyte cell line and mouse whole heart lysate
Blocking : 5%BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169