GeneTex
United States (US)

SNRPA antibody

GTX101664_40583_IHC_M_19011506_243.jpg
GTX101664_40583_IFA_19011506_172.jpg
GTX101664_40583_IHC_19011506_342.jpg
GTX101664_40583_IHC_R_19011506_932.jpg
GTX101664_40583_WB_19011506_234.jpg
GTX101664_40583_IHC_M_3_19011506_577.jpg
GTX101664_40583_IHC_M_2_19011506_752.jpg

Cat No. GTX101664

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SNRPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Small Nuclear Ribonucleoprotein Polypeptide A,Mud1,U1-A,U1A,Snrpa

Cellular Localization

Nucleus

Background

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq]

Database

DATA IMAGES
GTX101664_40583_IHC_M_19011506_243.jpg

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101664_40583_IFA_19011506_172.jpg

GTX101664 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SNRPA(GTX101664) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101664_40583_IHC_19011506_342.jpg

GTX101664 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using SNRPA(GTX101664) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101664_40583_IHC_R_19011506_932.jpg

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101664_40583_WB_19011506_234.jpg

GTX101664 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: HeLa
C: HepG2
12% SDS PAGE
GTX101664 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101664_40583_IHC_M_3_19011506_577.jpg

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101664_40583_IHC_M_2_19011506_752.jpg

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169