GeneTex
United States (US)

SNRPB antibody

GTX101883_39792_IFA_19011506_375.jpg
GTX101883_39792_WB_19011506_236.jpg

Cat No. GTX101883

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNRPB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COD antibody, SNRPB1 antibody, Sm-B/B' antibody, SmB/B' antibody, SmB/SmB' antibody, snRNP-B antibody, SNRPB antibody, small nuclear ribonucleoprotein polypeptide B antibody, small nuclear ribonucleoprotein-associated proteins B and B' antibody, small nuclear ribonucleoprotein polypeptides B and B' antibody, B polypeptide of Sm protein antibody, sm-B/Sm-B' antibody, Sm protein B/B' antibody, small nuclear ribonucleoprotein polypeptides B and B1 antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX101883_39792_IFA_19011506_375.jpg

GTX101883 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SNRPB(GTX101883) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101883_39792_WB_19011506_236.jpg

GTX101883 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX101883 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169