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SNRPB antibody

Anti-SNRPB antibody used in Western Blot (WB). GTX101883
Anti-SNRPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101883
Anti-SNRPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101883
Anti-SNRPB antibody used in Western Blot (WB). GTX101883
Anti-SNRPB antibody used in Western Blot (WB). GTX101883

Cat. No. GTX101883

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all SNRPB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNRPB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951963

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small nuclear ribonucleoprotein polypeptides B and B1 , CCMS , COD , SNRPB1 , Sm-B/B' , SmB/B' , SmB/SmB' , snRNP-B

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNRPB antibody used in Western Blot (WB). GTX101883

GTX101883 WB Image

Drosophila tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SNRPB antibody (GTX101883) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SNRPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101883

GTX101883 ICC/IF Image

SNRPB antibody detects SNRPB protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNRPB stained by SNRPB antibody (GTX101883) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-SNRPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101883

GTX101883 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SNRPB(GTX101883) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SNRPB antibody used in Western Blot (WB). GTX101883

GTX101883 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SNRPB antibody (GTX101883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNRPB antibody used in Western Blot (WB). GTX101883

GTX101883 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX101883 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

McGrail DJ et al. Cell Rep Med 2023; 4 (11) : 101255 Widespread BRCA1/2-independent homologous recombination defects are caused by alterations in RNA-binding proteins.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169