APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:2000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HeLa , HepG2 , HCT116
Predict Reactivity
Rat, Zebrafish, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.46 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SNRPD2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Small Nuclear Ribonucleoprotein D2 Polypeptide,Smd2,Snrpd1,Sm-D2,Snrpd2
Cellular Localization
Nucleus
Background
The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq]
Database
DATA IMAGES
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GTX101846 ICC/IF Image
SNRPD2 antibody detects SNRPD2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: SNRPD2 protein stained by SNRPD2 antibody (GTX101846) diluted at 1:2000.
Blue: Hoechst 33342 staining.
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GTX101846 IHC-P Image
SNRPD2 antibody detects SNRPD2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SNRPD2 antibody (GTX101846) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101846 WB Image
Sample (30 ug of whole cell lysate)
A: A549
15% SDS PAGE
GTX101846 diluted at 1:1000
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GTX101846 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SNRPD2(GTX101846) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101846 IHC-P Image
SNRPD2 antibody detects SNRPD2 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SNRPD2 antibody (GTX101846) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101846 IHC-P Image
SNRPD2 antibody detects SNRPD2 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNRPD2 antibody (GTX101846) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101846 IHC-P Image
SNRPD2 antibody detects SNRPD2 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SNRPD2 antibody (GTX101846) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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