GeneTex
United States (US)

SNRPN antibody

GTX114927_40240_WB_M_heart_18121410_762.jpg
GTX114927_40240_IHC_M_18121410_299.jpg
GTX114927_WB_18121410_384.jpg
GTX114927_40240_IHC_R_18121410_509.jpg

Cat No. GTX114927

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , mouse heart

Predict Reactivity

Bovine, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNRPN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Small Nuclear Ribonucleoprotein Polypeptide N,Hcern3,Pwcr,Rt-Li,Sm-D,Smn,Snrnp-N,Snurf-Snrpn,Sm-N,Snrpn

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. [provided by RefSeq]

Database

DATA IMAGES
GTX114927_40240_WB_M_heart_18121410_762.jpg

GTX114927 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX114927 diluted at 1:500

GTX114927_40240_IHC_M_18121410_299.jpg

GTX114927 IHC-P Image

SNRPN antibody detects SNRPN protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SNRPN antibody (GTX114927) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114927_WB_18121410_384.jpg

GTX114927 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
12% SDS PAGE
GTX114927 diluted at 1:1000

GTX114927_40240_IHC_R_18121410_509.jpg

GTX114927 IHC-P Image

SNRPN antibody detects SNRPN protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SNRPN antibody (GTX114927) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169