APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1 μg/ml |
| 1-2 μg/ml |
| Assay dependent |
| 0.5μg/10⁶ cells |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects alpha 1, beta 1 and beta 2 subunits of syntrophin. Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
syntrophin beta 2 , D16S2531E , EST25263 , SNT2B2 , SNT3 , SNTL
Cellular Localization
Membrane,Cell junction,Cytoplasm, cytoskeleton
Background
Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/templates/default/js/lightbox/images/loading.gif "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
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GTX11425 WB Image
WB analysis of canine lysate using GTX11425 SNTB2 antibody [1351].
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GTX11425 ICC/IF Image
ICC/IF analysis of PC-3 cells using GTX11425 SNTB2 antibody [1351]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX11425 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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GTX11425 WB Image
WB analysis of Syntrophin knockdown U87MG cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX11425 SNTB2 antibody [1351].
Dilution : 0.1-1 μg/ml
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GTX11425 WB Image
WB analysis of whole cell extracts (30 μg lysate) of U-87MG (Lane 1), PC 3 (Lane 2) and HeLa (Lane 3) using GTX11425 SNTB2 antibody [1351].
Dilution : 0.1-1 μg/ml
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GTX11425 ICC/IF Image
ICC/IF analysis of 293 cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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GTX11425 ICC/IF Image
ICC/IF analysis of A2058 Cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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REFERENCE
There are currently no references for SNTB2 antibody [1351] (GTX11425). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SNTB2 antibody [1351] (GTX11425). Be the first to share your experience with this product.
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_1471_WB_w_23060501_354.webp "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_234_ICC-IF_w_23060501_235.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_237_ICC-IF_w_23060501_127.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_1470_WB_w_23060501_265.webp "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_1472_WB_w_23060501_485.webp "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_235_ICC-IF_w_23060501_139.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/small/GTX11425/GTX11425_236_ICC-IF_w_23060501_292.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_1471_WB_w_23060501_354.webp "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_234_ICC-IF_w_23060501_235.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_237_ICC-IF_w_23060501_127.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_1470_WB_w_23060501_265.webp "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
![Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_1472_WB_w_23060501_485.webp "Anti-SNTB2 antibody [1351] used in Western Blot (WB). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_235_ICC-IF_w_23060501_139.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")
![Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425](/upload/website/prouct_img/normal/GTX11425/GTX11425_236_ICC-IF_w_23060501_292.webp "Anti-SNTB2 antibody [1351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11425")