APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 3-5μg/ml |
| 3-5μg/ml |
|
Note :
ICC/IF
Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y.
IHC-P
Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88278-PEP).
Product Note
This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2; NP_690039.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sorting nexin 1 , HsT17379 , VPS5
Cellular Localization
Endosome membrane
Background
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88278 IHC-P Image
IHC-P analysis of human uterus using GTX88278 SNX1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX88278 WB Image
WB analysis of HeLa (A) and U2OS (B) cell lysate (35μg protein in RIPA buffer) using GTX88278 SNX1 antibody, Internal.
Dilution : 1μg/ml (HeLa) and 0.1μg/ml (U2OS)
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GTX88278 WB Image
WB analysis of HeLa lysate using GTX88278 SNX1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88278 ICC/IF Image
ICC/IF analysis of formaldehyde-fixed SH-SY5Y using GTX88278 SNX1 antibody, Internal.
Dilution : 5μg/ml
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REFERENCE
There are currently no references for SNX1 antibody, Internal (GTX88278). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SNX1 antibody, Internal (GTX88278). Be the first to share your experience with this product.
