GeneTex
United States (US)

SNX1 antibody, Internal

GTX88278_130_ICC-IF_18122102_725.jpg
GTX88278_1027_IHC-P_18122102_375.jpg
GTX88278_2462_WB_18122102_890.jpg

Cat No. GTX88278

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF 3-5μg/ml
IHC-P 3-5μg/ml

Note :

ICC/IF
Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y.

IHC-P
Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium.

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88278-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1.

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2; NP_690039.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sorting Nexin 1,Hst17379,Vps5,Snx1

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88278_130_ICC-IF_18122102_725.jpg

GTX88278 ICC/IF Image

ICC/IF analysis of formaldehyde-fixed SH-SY5Y using GTX88278 SNX1 antibody, Internal.
Dilution : 5µg/ml

GTX88278_1027_IHC-P_18122102_375.jpg

GTX88278 IHC-P Image

IHC-P analysis of human uterus using GTX88278 SNX1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88278_2462_WB_18122102_890.jpg

GTX88278 WB Image

WB analysis of HeLa lysate using GTX88278 SNX1 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329