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SNX18 antibody [C3], C-term

KO/KD
Anti-SNX18 antibody [C3], C-term used in Western Blot (WB). GTX106319
Anti-SNX18 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106319
Anti-SNX18 antibody [C3], C-term used in Western Blot (WB). GTX106319

Cat. No. GTX106319

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SNX18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

H1299 , HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SNX18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951969

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorting nexin 18 , SH3PX2 , SH3PXD3B , SNAG1

Cellular Localization

Endomembrane system; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNX18 antibody [C3], C-term used in Western Blot (WB). GTX106319

GTX106319 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
GTX106319 diluted at 1:1000

Anti-SNX18 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106319

GTX106319 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using SNX18(GTX106319) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNX18 antibody [C3], C-term used in Western Blot (WB). GTX106319

GTX106319 WB Image

KO/KD

The data was published in the journal PLoS One in 2013. PMID: 23861900

REFERENCE

Application Reference

Ma MP et al. PLoS One 2013; 8 (7) : e68387 Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation.
Ma MP et al. J Cell Sci 2012; 125 (Pt 18) : 4372-82 SNX9, SNX18 and SNX33 are required for progression through and completion of mitosis.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169