APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 3-6μg/ml |
|
Note :
IHC-P
Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta .
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25983-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_055241.1; NP_001269383.1). Reported variants represent identical protein: NP_055241.1, NP_689413.1
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence SLLQSCIDLFKNN, from the C Terminus of the protein sequence according to NP_055241.1; NP_001269383.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sorting nexin 5
Cellular Localization
Endosome , Cell membrane , Cytoplasm
Background
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein functions in endosomal sorting, the phosphoinositide-signaling pathway, and macropinocytosis. This gene may play a role in the tumorigenesis of papillary thyroid carcinoma. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]
Database
Research Area
DATA IMAGES
|
GTX25983 IHC-P Image
IHC-P analysis of human breast using GTX25983 SNX5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
|
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GTX25983 WB Image
WB analysis of Jurkat lysate using GTX25983 SNX5 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for SNX5 antibody, C-term (GTX25983). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SNX5 antibody, C-term (GTX25983). Be the first to share your experience with this product.
