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SOAT1 antibody

Anti-SOAT1 antibody used in Western Blot (WB). GTX32890
Anti-SOAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32890
Anti-SOAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32890
Anti-SOAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32890

Cat. No. GTX32890

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SOAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SOAT1 (NP_003092.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sterol O-acyltransferase 1 , ACACT , ACAT , ACAT-1 , ACAT1 , SOAT , STAT

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-SOAT1 antibody used in Western Blot (WB). GTX32890

GTX32890 WB Image

WB analysis of various sample lysates using GTX32890 SOAT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SOAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32890

GTX32890 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32890 SOAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SOAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32890

GTX32890 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32890 SOAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SOAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32890

GTX32890 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32890 SOAT1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Ji Y et al. Nat Cell Biol 2023; 25 (5) : 726-739 SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399