APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1μg/ml |
| Assay dependent |
| Assay dependent |
| 10μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89995-PEP).
Predict Reactivity
Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-VLRDYLSSFPFQI, from the C Terminus of the protein sequence according to NP_003736.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
suppressor of cytokine signaling 1 , CIS1 , CISH1 , JAB , SOCS-1 , SSI-1 , SSI1 , TIP-3 , TIP3
Cellular Localization
Nucleus , Cytoplasmic vesicle
Background
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89995 IHC-P Image
IHC-P analysis of paraffin embedded human kidney using GTX89995 SOCS1 antibody, C-term.
Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 8μg/ml
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GTX89995 FACS Image
FACS analysis of PFA fixed HEK293 cells using GTX89995 SOCS1 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 1μg/ml
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GTX89995 IHC-P Image
IHC-P analysis of human kidney using GTX89995 SOCS1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89995 WB Image
WB analysis of human spleen (A), pig pancreas (B), (1ug/ml) mouse spleen (C) lysate and negative control HeLa (D) cell lysate using GTX89995 SOCS1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89995 ICC/IF Image
ICC/IF analysis of PFA fixed MCF7 cells using GTX89995 SOCS1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89995 IHC-P Image
IHC-P analysis of paraffin embedded mouse kidney using GTX89995 SOCS1 antibody, C-term.
Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 10μg/ml
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REFERENCE
There are currently no references for SOCS1 antibody, C-term (GTX89995). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SOCS1 antibody, C-term (GTX89995). Be the first to share your experience with this product.
