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SOCS1 antibody

Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657
Anti-SOCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100657
Anti-SOCS1 antibody used in Western Blot (WB). GTX100657
Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657
Anti-SOCS1 antibody used in Western Blot (WB). GTX100657

Cat No. GTX100657

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2 , mouse brain , *HOK , *DOK ,

Predict Reactivity

Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOCS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951971

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

suppressor of cytokine signaling 1 , CIS1 , CISH1 , JAB , SOCS-1 , SSI-1 , SSI1 , TIP-3 , TIP3

Background

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657

GTX100657 IHC-P Image

SOCS1 antibody detects SOCS1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SOCS1 antibody (GTX100657) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100657

GTX100657 ICC/IF Image

SOCS1 antibody detects SOCS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOCS1 protein stained by SOCS1 antibody (GTX100657) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SOCS1 antibody used in Western Blot (WB). GTX100657

GTX100657 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100657 diluted at 1:1000

Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657

GTX100657 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SOCS1(GTX100657) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOCS1 antibody used in Western Blot (WB). GTX100657

GTX100657 WB Image

SOCS1 antibody detects SOCS1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
SOCS1 antibody (GTX100657) dilution: 1:2000

REFERENCE

REVIEW

SOCS1 antibody Cat No. GTX100657

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HOK cells,DOK cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169