GeneTex
United States (US)

SOCS1 antibody

GTX100657_40051_IHC_M_19011506_894.jpg
GTX100657_40051_20160727_IFA_19011506_615.jpg
GTX100657_WB_19011506_864.jpg
GTX100657_IHC_19011506_298.jpg
GTX100657_39624_WB_M_brain_19011506_929.jpg

Cat No. GTX100657

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HepG2 , mouse brain , *HOK , *DOK ,

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOCS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Suppressor Of Cytokine Signaling 1,Cis1,Cish1,Jab,Socs-1,Ssi-1,Ssi1,Tip-3,Tip3,Socs1

Background

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100657_40051_IHC_M_19011506_894.jpg

GTX100657 IHC-P Image

SOCS1 antibody detects SOCS1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SOCS1 antibody (GTX100657) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100657_40051_20160727_IFA_19011506_615.jpg

GTX100657 ICC/IF Image

SOCS1 antibody detects SOCS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOCS1 protein stained by SOCS1 antibody (GTX100657) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100657_WB_19011506_864.jpg

GTX100657 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100657 diluted at 1:1000

GTX100657_IHC_19011506_298.jpg

GTX100657 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SOCS1(GTX100657) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100657_39624_WB_M_brain_19011506_929.jpg

GTX100657 WB Image

SOCS1 antibody detects SOCS1 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
SOCS1 antibody (GTX100657) dilution: 1:2000

REFERENCE
REVIEW

SOCS1 antibody Cat No. GTX100657

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HOK cells,DOK cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169