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SOCS1 antibody

Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657
Anti-SOCS1 antibody used in Western Blot (WB). GTX100657
Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657
Anti-SOCS1 antibody used in Western Blot (WB). GTX100657
Anti-SOCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100657

Cat. No. GTX100657

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SOCS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

mouse brain , HepG2

Predict Reactivity

Rat, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOCS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

suppressor of cytokine signaling 1 , CIS1 , CISH1 , JAB , SOCS-1 , SSI-1 , SSI1 , TIP-3 , TIP3

Background

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657

GTX100657 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SOCS1(GTX100657) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOCS1 antibody used in Western Blot (WB). GTX100657

GTX100657 WB Image

SOCS1 antibody detects SOCS1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
SOCS1 antibody (GTX100657) dilution: 1:2000

Anti-SOCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100657

GTX100657 IHC-P Image

SOCS1 antibody detects SOCS1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SOCS1 antibody (GTX100657) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOCS1 antibody used in Western Blot (WB). GTX100657

GTX100657 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100657 diluted at 1:1000

Anti-SOCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100657

GTX100657 ICC/IF Image

SOCS1 antibody detects SOCS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOCS1 protein stained by SOCS1 antibody (GTX100657) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

O. ERONAT et al. Eur Rev Med Pharmacol Sci 2023; 27 (14) : 6639-6652 Increased SOCS1 and SOCS3 expression in papillary thyroid carcinoma and its association with prognosis
Application : IHC-P
Reactivity : Human
Chang CJ et al. Biochem Pharmacol 2021; 183 : 114356 Overcoming interferon (IFN)-γ resistance ameliorates transforming growth factor (TGF)-β-mediated lung fibroblast-to-myofibroblast transition and bleomycin-induced pulmonary fibrosis.
Application : WB
Reactivity : Human
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Wang J et al. Oncoimmunology 2016; 5 (11) : e1232223 microRNA-155 deficiency impairs dendritic cell function in breast cancer.
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REVIEW

SOCS1 antibody Cat. No. GTX100657

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-SOCS1 antibody used in Western Blot (WB). GTX100657
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HOK cells,DOK cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169