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SOCS2 antibody

Anti-SOCS2  antibody used in Western Blot (WB). GTX23692
Anti-SOCS2  antibody used in Immunohistochemistry (IHC). GTX23692

Cat No. GTX23692

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($339)

APPLICATION

Application Note

Immunoprecipitation: use at a concentration of 3.0 - 5.0 μg / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5 - 2.0 μg/ml. ELISA: use at a concentration of 0.1 - 1.0 μg/ml. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 23 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 2. Samples wereboiled prior to subject 10% SDS-polyacrylamide gelelectrophoresis.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (PTRLKDYLEEYKFQV) correspondingto amino acids 184-198 of human, rat and mouse SOCS 2.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Suppressor Of Cytokine Signaling 2 , Cis2 , Cish2 , Socs-2 , Ssi-2 , Ssi2 , Stati2 , Socs2

Background

Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.

Database

Research Area

DATA IMAGES

Anti-SOCS2  antibody used in Western Blot (WB). GTX23692

GTX23692 WB Image

Western blot analysis of mouse spleen tissue cell lysate probed with GTX23692 at 2ug/ml.

Anti-SOCS2  antibody used in Immunohistochemistry (IHC). GTX23692

GTX23692 IHC Image

Localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain in cardiac myocytes of the heart.

REFERENCE

REVIEW

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Package List Price ($)
$ 339