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SOCS3 antibody [N1C3]

Comparison Orthogonal Overexpression
Anti-SOCS3 antibody [N1C3] used in Western Blot (WB). GTX113049
Anti-SOCS3 antibody [N1C3] used in Western Blot (WB). GTX113049

Cat. No. GTX113049

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all SOCS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Raw264.7 (50 ng/ml LPS treatment for 4 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOCS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722297

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

suppressor of cytokine signaling 3 , ATOD4 , CIS3 , Cish3 , SOCS-3 , SSI-3 , SSI3

Background

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOCS3 antibody [N1C3] used in Western Blot (WB). GTX113049

GTX113049 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOCS3 antibody [N1C3] (GTX113049) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SOCS3 antibody [N1C3] used in Western Blot (WB). GTX113049

GTX113049 WB Image

Comparison Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with SOCS3 antibody [N1C3] (GTX113049) diluted at 1:10000 and competitor's antibody (#52113) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Ho CH et al. Int J Med Microbiol 2020; 310 (7) : 151450 Testosterone regulates the intracellular bacterial community formation of uropathogenic Escherichia coli in prostate cells via STAT3.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169