GeneTex
  • Country / Location Selection

United States (US)

SOD1 antibody [HL1652]

Anti-SOD1 antibody [HL1652] used in Western Blot (WB). GTX637250
Anti-SOD1 antibody [HL1652] used in IHC (Paraffin sections) (IHC-P). GTX637250
Anti-SOD1 antibody [HL1652] used in Western Blot (WB). GTX637250
Anti-SOD1 antibody [HL1652] used in IHC (Paraffin sections) (IHC-P). GTX637250
Anti-SOD1 antibody [HL1652] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637250
Anti-SOD1 antibody [HL1652] used in Western Blot (WB). GTX637250

Cat. No. GTX637250

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1652

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

Cellular Localization

Cytoplasm,Mitochondrion,Nucleus

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOD1 antibody [HL1652] used in Western Blot (WB). GTX637250

GTX637250 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (GTX637250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody [HL1652] used in IHC (Paraffin sections) (IHC-P). GTX637250

GTX637250 IHC-P Image

SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD1 antibody [HL1652] used in Western Blot (WB). GTX637250

GTX637250 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (GTX637250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody [HL1652] used in IHC (Paraffin sections) (IHC-P). GTX637250

GTX637250 IHC-P Image

SOD1 antibody [HL1652] detects SOD1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD1 antibody [HL1652] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637250

GTX637250 ICC/IF Image

SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SOD1 antibody [HL1652] used in Western Blot (WB). GTX637250

GTX637250 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (GTX637250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for SOD1 antibody [HL1652] (GTX637250). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SOD1 antibody [HL1652] (GTX637250). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations