SOD1 antibody [HL1652]

Cat. No. GTX637250

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1652
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all SOD1 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

Cellular Localization

Cytoplasm,Mitochondrion,Nucleus

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6647 SOD1
UniProt: P00441 SOD1

Research Area

DATA IMAGES

GTX637250 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (GTX637250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637250 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (GTX637250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637250 ICC/IF Image

SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637250 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1652] (GTX637250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637250 IHC-P Image

SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637250 IHC-P Image

SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637250 IHC-P Image

SOD1 antibody [HL1652] detects SOD1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637250 IHC-P Image

SOD1 antibody [HL1652] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

SOD1 antibody [HL1652]

Cat. No. GTX637250

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX637250 WB Image

GTX637250 WB Image

GTX637250 ICC/IF Image

GTX637250 WB Image

GTX637250 IHC-P Image

GTX637250 IHC-P Image

GTX637250 IHC-P Image

GTX637250 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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