APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89049-PEP).
Predict Reactivity
Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer
Cellular Localization
Cytoplasm , Mitochondrion , Nucleus
Background
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89049 ELISA Image
ELISA analysis of SOD1 protein using GTX89049 SOD1 antibody, Internal.
Capture : GTX100554 (5μg/ml)
Detection : GTX89049 (1.5μg/ml)
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GTX89049 WB Image
WB analysis of mouse brain lysate using GTX89049 SOD1 antibody, Internal.
Dilution : 0.02μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89049 WB Image
WB analysis of NIH-3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates using GTX89049 SOD1 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for SOD1 antibody, Internal (GTX89049). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SOD1 antibody, Internal (GTX89049). Be the first to share your experience with this product.
