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SOD1 antibody

Anti-SOD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100554
Anti-SOD1 antibody used in Western Blot (WB). GTX100554
Anti-SOD1 antibody used in Western Blot (WB). GTX100554
Anti-SOD1 antibody used in Western Blot (WB). GTX100554
Anti-SOD1 antibody used in Western Blot (WB). GTX100554
Anti-SOD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100554
Anti-SOD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100554
Anti-SOD1 antibody used in ELISA (ELISA). GTX100554
Anti-SOD1 antibody used in Western Blot (WB). GTX100554

Cat No. GTX100554

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, sELISA
Reactivity Human, Mouse, Rat, Alligator, Bird
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX100554 , Detection: GTX89049

Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , HeLa , NT2D1 , SK-N-AS , IMR32 , SK-N-SH , U87-MG , mouse brain , Rat brain , *F79 , *F123

Predict Reactivity

Rabbit, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

superoxide dismutase 1,ALS,ALS1,HEL-S-44,IPOA,SOD,hSod1,homodimer

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SOD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100554

GTX100554 IHC-P Image

Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain.
Green: SOD1 antibody (GTX100554) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SOD1 antibody used in Western Blot (WB). GTX100554

GTX100554 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100554

GTX100554 WB Image

SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100554

GTX100554 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100554

GTX100554 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100554

GTX100554 IHC-P Image

SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SOD1 antibody (GTX100554) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SOD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100554

GTX100554 ICC/IF Image

SOD1 antibody detects SOD1 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 protein stained by SOD1 antibody (GTX100554) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

Anti-SOD1 antibody used in ELISA (ELISA). GTX100554

GTX100554 ELISA Image

ELISA detection of SOD1 using GTX100554 for capture at a concentration of 5 µg/mL and GTX89049 for detection at a concentration of 1.5 µg/mL.

Anti-SOD1 antibody used in Western Blot (WB). GTX100554

GTX100554 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

SOD1 antibody Cat No. GTX100554

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 22-Apr-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : F79 cells,F123 cells
Amount used : 40μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169