GeneTex
United States (US)

SOD1 antibody

KO/KD
GTX100554_40163_WB_shRNA_watermark_19011506_273.jpg
GTX100554_40163_20150414_IHC-P_M_19011506_641.jpg
GTX100554_40163_20160520_IFA_19011506_495.jpg
SOD1_ELISA-PAIR_19011506_991.jpg
GTX100554_40163_WB_R_Brain_19011506_678.jpg
GTX100554_40163_20150313_WB_2_19011506_188.jpg
GTX100554_43222_20180601_WB_19021719_743.jpg
GTX100554_40163_20160526_WB_shRNA_watermark_19011506_727.jpg
GTX100554_40163_WB_M_Brain_19011506_415.jpg
GTX100554_40163_IHC_M_19011506_605.jpg

Cat No. GTX100554

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat, Alligator, Bird
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , HeLa , NT2D1 , SK-N-AS , IMR32 , SK-N-SH , U87-MG , mouse brain , Rat brain , *F79 , *F123

Predict Reactivity

Rabbit, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALS antibody, ALS1 antibody, IPOA antibody, SOD antibody, homodimer antibody, SOD1 antibody, "superoxide dismutase, cystolic antibody", Cu/Zn superoxide dismutase antibody, indophenoloxidase A antibody, superoxide dismutase [Cu-Zn] antibody, "SOD, soluble antibody", "superoxide dismutase 1, soluble antibody"

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100554_40163_WB_shRNA_watermark_19011506_273.jpg

GTX100554 WB Image

KO/KD

Western blot analysis of SOD1 (GTX100554, upper panel) and beta-actin (GTX109639, lower panel) 
Sample (10 µg of whole cell lysate) 
A: HeLa mock control 
B: HeLa transfected shSOD1
15% SDS PAGE 
GTX100554 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100554_40163_20150414_IHC-P_M_19011506_641.jpg

GTX100554 IHC-P Image

Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain.
Green: SOD1 antibody (GTX100554) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100554_40163_20160520_IFA_19011506_495.jpg

GTX100554 ICC/IF Image

SOD1 antibody detects SOD1 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 protein stained by SOD1 antibody (GTX100554) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

SOD1_ELISA-PAIR_19011506_991.jpg

GTX100554 ELISA Image

ELISA detection of SOD1 using GTX100554 for capture at a concentration of 5 µg/mL and GTX89049 for detection at a concentration of 1.5 µg/mL.

GTX100554_40163_WB_R_Brain_19011506_678.jpg

GTX100554 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100554_40163_20150313_WB_2_19011506_188.jpg

GTX100554 WB Image

SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100554_43222_20180601_WB_19021719_743.jpg

GTX100554 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100554_40163_20160526_WB_shRNA_watermark_19011506_727.jpg

GTX100554 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100554_40163_WB_M_Brain_19011506_415.jpg

GTX100554 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100554_40163_IHC_M_19011506_605.jpg

GTX100554 IHC-P Image

SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SOD1 antibody (GTX100554) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

SOD1 antibody Cat No. GTX100554

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 22-Apr-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : F79 cells,F123 cells
Amount used : 40μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169