GeneTex
United States (US)

SOD1 antibody

KO/KD
GTX100659_39988_WB_shRNA_watermark_19011506_188.jpg
GTX100659_39988_IHC_M_19011506_310.jpg
GTX100659_39988_IHC_19011506_955.jpg
GTX100659_39988_WB_R_Brain_19011506_659.jpg
GTX100659_39988_IHC_R_19011506_796.jpg
GTX100659_41822_20160526_WB_shRNA_watermark_19011506_235.jpg
GTX100659_39988_20150410_IFA_19011506_863.jpg
GTX100659_39988_WB_19011506_562.jpg
GTX100659_41822_20141128_WB_19011506_812.jpg

Cat No. GTX100659

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NT2D1 , PC-3 , SK-N-SH , Rat brain , *U87-MG

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALS antibody, ALS1 antibody, IPOA antibody, SOD antibody, homodimer antibody, SOD1 antibody, superoxide dismutase, cystolic antibody, Cu/Zn superoxide dismutase antibody, indophenoloxidase A antibody, superoxide dismutase [Cu-Zn] antibody, SOD, soluble antibody, superoxide dismutase 1, soluble antibody

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100659_39988_WB_shRNA_watermark_19011506_188.jpg

GTX100659 WB Image

KO/KD

Western blot analysis of SOD1 (GTX100659, upper panel) and beta-actin (GTX109639, lower panel) 
Sample (30 µg of whole cell lysate) 
A: HeLa mock control 
B: HeLa transfected shSOD1
15% SDS PAGE 
GTX100659 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100659_39988_IHC_M_19011506_310.jpg

GTX100659 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using SOD1(GTX100659) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100659_39988_IHC_19011506_955.jpg

GTX100659 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SOD1(GTX100659) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100659_39988_WB_R_Brain_19011506_659.jpg

GTX100659 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100659 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100659_39988_IHC_R_19011506_796.jpg

GTX100659 IHC-P Image

Immunohistochemical analysis of paraffin-embedded rat brain, using SOD1(GTX100659) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100659_41822_20160526_WB_shRNA_watermark_19011506_235.jpg

GTX100659 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100659_39988_20150410_IFA_19011506_863.jpg

GTX100659 ICC/IF Image

SOD1 antibody detects SOD1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 protein stained by SOD1 antibody (GTX100659) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100659_39988_WB_19011506_562.jpg

GTX100659 WB Image

SOD1 antibody detects SOD1 protein by western blot analysis.
A.30 µg NT2D1 whole cell lysate/extract
B.30 µg PC-3 whole cell lysate/extract
C.30 µg U87-MG whole cell lysate/extract
D.30 µg SK-N-SH whole cell lysate/extract
15% SDS-PAGE
SOD1 antibody (GTX100659) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100659_41822_20141128_WB_19011506_812.jpg

GTX100659 WB Image

SOD1 antibody detects SOD1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

SOD1 antibody Cat No. GTX100659

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169