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SOD1 (mutant) antibody [MS27]

Anti-SOD1 (mutant) antibody [MS27] used in Immunoprecipitation (IP). GTX57212
Anti-SOD1 (mutant) antibody [MS27] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57212
Anti-SOD1 (mutant) antibody [MS27] used in Immunoprecipitation (IP). GTX57212

Cat. No. GTX57212

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MS27

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Human
Package
200 μl ($409)
See all SOD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1 μg/ml (1/500 dilution) -5 μg/ml (1/100 dilution)
IP 1-5μg/sample
ELISA Assay dependent
IHC 1 μg/ml (1/500 dilution) -5 μg/ml (1/100 dilution)

Note :

ICC/IF
Target : SOD1 mutants

IP
Target : SOD1 mutants under native condition ; Wild-type SOD1 under the zinc-deficient ER-stress. GTX57211 and GTX57212 could recognize not only mutants but also denatured SOD1. Please keep non-denatured condition and avoid to use high concentration of SDS or the other denaturing compounds.

IHC
Target : SOD1 mutants
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

Clone MS785 and MS27, which specifically bind to conformationally altered SOD1 mutants, not detecting wild type SOD1 homo-dimer. Both clones are succeeded in specifically detecting over 100 SOD1 mutants (please see to Appendix for detail description).

PROPERTIES

Form

Liquid

Buffer

1x PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human SOD1 30-40 amino acids.

Purification

Protein G Purified.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Superoxide Dismutase 1 , Als , Als1 , Hel-S-44 , Ipoa , Sod , Hsod1 , Homodimer , Sod1

Research Area

DATA IMAGES

Anti-SOD1 (mutant) antibody [MS27] used in Immunoprecipitation (IP). GTX57212

GTX57212 IP Image

IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”.
Antibody amount :
MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg
IP lysis buffer: 1% Triton X100/TBS buffer.

Anti-SOD1 (mutant) antibody [MS27] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57212

GTX57212 ICC/IF Image

ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail.
Fixation : 4% PFA for 10 min at RT.
Permeabilization : 0.2% Triton X-100 for 5 min
Dilution : 1μg/ml (12 hours at 4ºC)

Anti-SOD1 (mutant) antibody [MS27] used in Immunoprecipitation (IP). GTX57212

GTX57212 IP Image

IP analysis of HEK293 cells cultured in the presence and absence of 10μM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation.
Antibody amount : 1-5 μg

REFERENCE

Clone Reference

Fujisawa T et al. Neurobiol Dis 2015; 82:478-486 A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants.
Application : WB, ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Document
GTX57212_Appendix.pdf
Package List Price ($)
$ 409