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SOD1 (mutant) antibody [MS785]

Anti-SOD1 (mutant) antibody [MS785] used in Immunoprecipitation (IP). GTX57211
Anti-SOD1 (mutant) antibody [MS785] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57211
Anti-SOD1 (mutant) antibody [MS785] used in Immunoprecipitation (IP). GTX57211

Cat. No. GTX57211

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MS785

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Human
Package
200 μl ($409)
See all SOD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1 μg/ml (1/500 dilution) -5 μg/ml (1/100 dilution)
IP 1-5μg/sample
ELISA Assay dependent
IHC 1 μg/ml (1/500 dilution) -5 μg/ml (1/100 dilution)

Note :

ICC/IF
Target : SOD1 mutants

IP
Target : SOD1 mutants under native condition ; Wild-type SOD1 under the zinc-deficient ER-stress. GTX57211 and GTX57212 could recognize not only mutants but also denatured SOD1. Please keep non-denatured condition and avoid to use high concentration of SDS or the other denaturing compounds.

IHC
Target : SOD1 mutants
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

Clone MS785 and MS27, which specifically bind to conformationally altered SOD1 mutants, not detecting wild type SOD1 homo-dimer. Both clones are succeeded in specifically detecting over 100 SOD1 mutants (please see to Appendix for detail description).

PROPERTIES

Form

Liquid

Buffer

1x PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human SOD1 8-14 amino acids.

Purification

Protein G Purified.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Superoxide Dismutase 1 , Als , Als1 , Hel-S-44 , Ipoa , Sod , Hsod1 , Homodimer , Sod1

Research Area

DATA IMAGES

Anti-SOD1 (mutant) antibody [MS785] used in Immunoprecipitation (IP). GTX57211

GTX57211 IP Image

IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”.
Antibody amount :
MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg
IP lysis buffer: 1% Triton X100/TBS buffer.

Anti-SOD1 (mutant) antibody [MS785] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57211

GTX57211 ICC/IF Image

ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail.
Fixation : 4% PFA for 10 min at RT.
Permeabilization : 0.2% Triton X-100 for 5 min
Dilution : 1μg/ml (12 hours at 4ºC)

Anti-SOD1 (mutant) antibody [MS785] used in Immunoprecipitation (IP). GTX57211

GTX57211 IP Image

IP analysis of HEK293 cells cultured in the presence and absence of 10μM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation.
Antibody amount : 1-5 μg

REFERENCE

Clone Reference

Fujisawa T et al. Neurobiol Dis 2015; 82:478-486 A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants.
Application : WB, ICC/IF
Reactivity : Human
Fujisawa T et al. Ann Neurol 2012; 72 (5):739-49 A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Document
GTX57211_Appendix.pdf
Package List Price ($)
$ 409