GeneTex
United States (US)

SOD2 antibody [1H6]

GTX31147_1347_IHC-P_19011519_731.jpg
GTX31147_1855_WB_19011519_768.jpg
GTX31147_763_ICC-IF_19011519_721.jpg
GTX31147_765_ICC-IF_19011519_173.jpg
GTX31147_764_ICC-IF_19011519_744.jpg
GTX31147_1346_IHC-P_19011519_154.jpg
GTX31147_1453_IP_19011519_287.jpg
GTX31147_1856_WB_19011519_204.jpg
GTX31147_1348_IHC-P_19011519_456.jpg
GTX31147_1349_IHC-P_19011519_774.jpg

Cat No. GTX31147

Host Mouse
Clonality Monoclonal
Clone Name 1H6
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/mL
ICC/IF 2 μg/ml
IHC-P 1:100
IP 2 μg
Not tested in other applications.

Calculated MW

25 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant protein of human SOD2

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Superoxide Dismutase 2,Ipo-B,Ipob,Mnsod,Mvcd6,Mn-Sod,Sod2

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES
GTX31147_1347_IHC-P_19011519_731.jpg

GTX31147 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31147_1855_WB_19011519_768.jpg

GTX31147 WB Image

WB analysis of 50 ug of various samples using GTX31147 SOD2 antibody [1H6].
Dilution : 1:500

GTX31147_763_ICC-IF_19011519_721.jpg

GTX31147 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 µg/ml in 0.1% BSA incubated for 3 hours at room temperature

GTX31147_765_ICC-IF_19011519_173.jpg

GTX31147 ICC/IF Image

ICC/IF analysis of HUVEC cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 °C in a humidified chamber

GTX31147_764_ICC-IF_19011519_744.jpg

GTX31147 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 °C in a humidified chamber

GTX31147_1346_IHC-P_19011519_154.jpg

GTX31147 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31147_1453_IP_19011519_287.jpg

GTX31147 IP Image

IP analysis of HepG2 cell lysates using GTX31147 SOD2 antibody [1H6].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:500

GTX31147_1856_WB_19011519_204.jpg

GTX31147 WB Image

WB analysis of whole cell extracts (30 µg lysate) of HepG2 (Lane 1), Jurkat (Lane 2), K562 (Lane 3), HeLa (Lane 4), U-87 MG (Lane 5), COLO 205 (Lane 6), HEK293 (Lane 7) and HUVEC (Lane 8) using GTX31147 SOD2 antibody [1H6].
Dilution : 1-2 µg/ml

GTX31147_1348_IHC-P_19011519_456.jpg

GTX31147 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

GTX31147_1349_IHC-P_19011519_774.jpg

GTX31147 IHC-P Image

IHC-P analysis of human liver tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200