IHC-P analysis of human liver tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

ICC/IF analysis of HUVEC cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC in a humidified chamber

WB analysis of 50 ug of various samples using GTX31147 SOD2 antibody [1H6].
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC in a humidified chamber

IHC-P analysis of human cervical carcinoma tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

IP analysis of HepG2 cell lysates using GTX31147 SOD2 antibody [1H6].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

IHC-P analysis of human cerebellum tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

WB analysis of whole cell extracts (30 μg lysate) of HepG2 (Lane 1), Jurkat (Lane 2), K562 (Lane 3), HeLa (Lane 4), U-87 MG (Lane 5), COLO 205 (Lane 6), HEK293 (Lane 7) and HUVEC (Lane 8) using GTX31147 SOD2 antibody [1H6].
Dilution : 1-2 μg/ml

IHC-P analysis of human kidney tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100